My first protein gel: Mein erstes Proteingel:
And not that bad. All three proteins were expressed, and one is also present in the soluble sample. For those who don't know what I am speaking about: we have isolated all Proteins from some E.coli cells and then loaded onto an Polyacrylamidgel. The proteins are denaturated and are seperated regarding their molecular weight(similar to DNA, small proteins are faster). The dark bands are the recombinant proteins. Und garnichtmal so schlecht. Alle drei Protein wurden erfolgreich exprimiert und eins ist sogar löslich. Für die die nicht wissen wovon ich rede: wir haben aus den E.colis die wir angezogen haben mit dem entsprechenden fremden gen alle Proteine isoliert und auf ein Polyacrylamidgel aufgetragen. Die Proteine sind denaturiert und werden somit nach ihrem Molekulargewicht aufgetrennt. Dabei sind wie bei der DNA die kleinen Proteine schneller. Die dicken schwarzen banden sind die rekombinanten Proteine.
Some more lab-arts without comment:
Shit, the washer gives no change and you need 1,80 Pound (expensive, like everything). Just drop into edge and ask the waiter for change. Back to the laundry. Insert coins, hmm it doesn't take 5p, so I had to insert 1,90 Pound. 45 minutes later I took the clothes out, they seem to be clean, at least that....The the next challenge, the dryer. I don't want my clothes to become smaller so I wanted to try it for 20 minutes at first. But the dryer doesn't start with less than 1Pound. ok, after 20minutes I took the socks and the T-Shirts out, but they did not seem to be dryer than before. ok, I hoped at least the towels would be dry after the full 50 minutes. But that would have been too nice... so it looks like this in my bathroom:
